Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial (Active)
Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial (Active)
Cat：
GS13193
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Type：
Protein
Alias：
Expressed Host：
Mammalian cell
Species：
Homo sapiens (Human)
Price：
＄
Data Sheet
MSDS
  • Product details
Product Name
Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial (Active)
Cat
GS13193
Expression Region
Uniorot ID:Q9BYF1(18-740aa)
Alias
Expressed Host
Mammalian cell
Species
Homo sapiens (Human)
Tag
C-terminal hFc-tagged
Purity
Greater than 85% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Buffer
Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Biological activity
①Measured by ACE2 protein (CSB-MP866317HU) binding ability in a functional ELISA. Coat the plate with 0.2μg/well (2μg/ml, 100μl/well) of SARS-CoV-2 S1 protein (CSB-MP3324GMY), add 100μl/well (0.7629-10ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific (CSB-PA160506). Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1 protein could bind ACE2 protein. The EC50 is 56.64 - 103.6 ng/ml. ②Measured by ACE2 protein (CSB-MP866317HU) binding ability in a functional ELISA. Coat the plate with 0.5μg/well (5μg/ml, 100μl/well) of SARS-CoV-2 S1-RBD protein (CSB-YP3324GMY1), add 100μl/well (0.762939-10ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific (CSB-PA160506). Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 31.80 - 44.69 ng/ml. ③Measured by ACE2 protein (CSB-MP866317HU) binding ability in a functional ELISA. Coat the plate with 0.5μg/well (5μg/ml, 100μl/well) of SARS-CoV-2 S1-RBD protein (CSB-MP3324GMY1b1), add 100μl/well (0.762939-10ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific (CSB-PA160506). Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 2.785-9.139 ng/ml. ④Measured by ACE2 protein (CSB-MP866317HU) binding ability in a functional ELISA. Coat the plate with 0.2μg/well (2μg/ml, 100μl/well) of ACE2 protein, add 100μl/well (0.1164153-20ng/ml, gradient dilution, 4x to 18x) of SARS-CoV-2 S1-RBD protein (CSB-MP3324GMY1) and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific (CSB-PA160506). Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 8.363-12.82 ng/ml ng/ml. ⑤The SPR sensorgram shows the binding between ACE2 protein (CSB-MP866317HU) and SARS-CoV-2 S1-RBD protein (CSB-YP3324GMY1). ACE2 protein was injected at 5 concentrations (400, 200, 100, 50, 0 nM) across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 100 nM. ⑥The SPR sensorgram shows the binding between ACE2 protein (CSB-MP866317HU) and SARS-CoV-2 S1-RBD protein (CSB-MP3324GMY1b1). ACE2 protein was injected at 5 concentrations (100, 50, 25, 12.5, 0 nM) across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 13.8 nM.
Storage
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Type
Protein
Purposes
For research use only
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