Recombinant Human Interleukin-6 protein(IL6) (Active)
Recombinant Human Interleukin-6 protein(IL6) (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human Interleukin-6 protein(IL6) (Active)
Cat：
GS12642
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Type：
Protein
Alias：
IL-6, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor
Expressed Host：
E.Coli
Species：
Homo sapiens (Human)
Price：
＄
Data Sheet
MSDS
  • Product details
Product Name
Recombinant Human Interleukin-6 protein(IL6) (Active)
Cat
GS12642
Expression Region
Uniorot ID:P05231(30-212aa)
Alias
IL-6, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor
Expressed Host
E.Coli
Species
Homo sapiens (Human)
Tag
Tag-Free
Purity
>96% as determined by SDS-PAGE and HPLC.
Endotoxin
Less than 1.0 EU/µg as determined by LAL method.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Biological activity
Assay #1: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. Assay #2: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine T1165 cells is less than 0.8 ng/ml, corresponding to a specific activity of > 1.25 ×106 IU/mg.
Storage
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Type
Protein
Purposes
For research use only
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