Recombinant Human NAD-dependent malic enzyme, mitochondrial protein(ME2) (Active)
Recombinant Human NAD-dependent malic enzyme, mitochondrial protein(ME2) (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human NAD-dependent malic enzyme, mitochondrial protein(ME2) (Active)
Cat：
GS12882
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Type：
Protein
Alias：
NAD-ME, Malic enzyme 2
Expressed Host：
E.Coli
Species：
Homo sapiens (Human)
Price：
＄
Data Sheet
MSDS
  • Product details
Product Name
Recombinant Human NAD-dependent malic enzyme, mitochondrial protein(ME2) (Active)
Cat
GS12882
Expression Region
Uniorot ID:P23368(19-584aa)
Alias
NAD-ME, Malic enzyme 2
Expressed Host
E.Coli
Species
Homo sapiens (Human)
Tag
C-terminal 6xHis-tagged
Purity
>95% as determined by SDS-PAGE and HPLC.
Endotoxin
Less than 1.0 EU/µg as determined by LAL method.
Buffer
Lyophilized from a 0.2μm filtered PBS, pH 7.4.
Biological activity
Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 ± 0.6 mM
Storage
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Type
Protein
Purposes
For research use only
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