Recombinant Human Nectin-4(NECTIN4),partial (Active)
Recombinant Human Nectin-4(NECTIN4),partial (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human Nectin-4(NECTIN4),partial (Active)
Cat:
GS13114
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Type:
Protein
Alias:
PVRL4;Nectin-4;Ig superfamily receptor LNIR;Poliovirus receptor-related protein 4;PRR4;LNIR
Expressed Host:
Mammalian cell
Species:
Homo sapiens (Human)
Price:
  • Product details
Product Name
Recombinant Human Nectin-4(NECTIN4),partial (Active)
Cat
GS13114
Expression Region
Uniorot ID:Q96NY8(32-351aa)
Alias
PVRL4;Nectin-4;Ig superfamily receptor LNIR;Poliovirus receptor-related protein 4;PRR4;LNIR
Expressed Host
Mammalian cell
Species
Homo sapiens (Human)
Tag
C-terminal 6xHis-tagged
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/µg as determined by LAL method.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.4
Biological activity
Specific activity as determined by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells is greater than 40%. When 5 x 10^4 cells/well are added to plates coated with recombinant human Nectin-4 (1 µg/mL with 100 µL/well), approximately >50% will adhere after 40 min incubation at 37 °C.
Storage
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Type
Protein
Purposes
For research use only