Recombinant Human Noggin protein(NOG) (Active)
Recombinant Human Noggin protein(NOG) (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human Noggin protein(NOG) (Active)
Cat:
GS12844
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Type:
Protein
Alias:
Expressed Host:
E.Coli
Species:
Homo sapiens (Human)
Price:
  • Product details
Product Name
Recombinant Human Noggin protein(NOG) (Active)
Cat
GS12844
Expression Region
Uniorot ID:Q13253(M+28-232aa)
Alias
Expressed Host
E.Coli
Species
Homo sapiens (Human)
Tag
Tag-Free
Purity
>95% as determined by SDS-PAGE and HPLC.
Endotoxin
Less than 1.0 EU/µg as determined by LAL method.
Buffer
Lyophilized from a 0.2 μm filtered 30 % acetonitrile, 0.1 % TFA
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by inhibiting BMP-4- induced alkaline phosphatase production of murine ATDC5 cells is less than 3.0 ng/ml, corresponding to a specific activity of > 3.3 × 105 IU/mg in the presence of 5 ng/ml rHuBMP-4.
Storage
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Type
Protein
Purposes
For research use only