Recombinant Human Novel Coronavirus Spike glycoprotein(S),partial (Active)
Recombinant Human Novel Coronavirus Spike glycoprotein(S),partial (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human Novel Coronavirus Spike glycoprotein(S),partial (Active)
Cat:
GS13179
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Type:
Protein
Alias:
Expressed Host:
Mammalian cell
Species:
Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Price:
  • Product details
Product Name
Recombinant Human Novel Coronavirus Spike glycoprotein(S),partial (Active)
Cat
GS13179
Expression Region
Uniorot ID:P0DTC2(16-685aa)
Alias
Expressed Host
Mammalian cell
Species
Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Tag
N-terminal 10xHis-tagged and C-terminal Flag-tagged
Purity
Greater than 85% as determined by SDS-PAGE.
Endotoxin
Not test.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Biological activity
①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S at 2 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 of SARS-CoV-2-S protein is 56.64 - 103.6 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A1GMY), the EC50 of SARS-CoV-2-S protein is 36.79-48.87 ng/ml.
Storage
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Type
Protein
Purposes
For research use only