Recombinant Human Programmed cell death protein 1(PDCD1),partial (Active)
Recombinant Human Programmed cell death protein 1(PDCD1),partial (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human Programmed cell death protein 1(PDCD1),partial (Active)
Cat：
GS13166
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Type：
Protein
Alias：
(Protein PD-1) (hPD-1) (CD279)
Expressed Host：
Mammalian cell
Species：
Homo sapiens (Human)
Price：
＄
Data Sheet
MSDS
  • Product details
Product Name
Recombinant Human Programmed cell death protein 1(PDCD1),partial (Active)
Cat
GS13166
Expression Region
Uniorot ID:Q15116(25-167aa)
Alias
(Protein PD-1) (hPD-1) (CD279)
Expressed Host
Mammalian cell
Species
Homo sapiens (Human)
Tag
C-terminal 6xHis-tagged
Purity
Greater than 93% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Biological activity
①Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 μg/ml can bind Anti-PD-1 recombinant antibody, the EC50 of human PD-1 protein is 6.087-7.854 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 μg/ml can bind Nivolumab, the EC50 of human PD-1 protein is 9.713-12.39 ng/ml.
Storage
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Type
Protein
Purposes
For research use only
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