Recombinant Human SPARC protein(SPARC) (Active)
Recombinant Human SPARC protein(SPARC) (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human SPARC protein(SPARC) (Active)
Cat：
GS12893
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Type：
Protein
Alias：
Basement-membrane protein 40, Osteonectin, ON, Secreted protein acidic and rich in cysteine,
Expressed Host：
E.Coli
Species：
Homo sapiens (Human)
Price：
＄
Data Sheet
MSDS
  • Product details
Product Name
Recombinant Human SPARC protein(SPARC) (Active)
Cat
GS12893
Expression Region
Uniorot ID:P09486(18-303aa)
Alias
Basement-membrane protein 40, Osteonectin, ON, Secreted protein acidic and rich in cysteine,
Expressed Host
E.Coli
Species
Homo sapiens (Human)
Tag
N-terminal 6xHis-tagged
Purity
>95% as determined by SDS-PAGE and HPLC.
Endotoxin
Less than 1.0 EU/µg as determined by LAL method.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 µg/mL, corresponding to a specific activity of > 333 IU/mg.
Storage
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Type
Protein
Purposes
For research use only
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