Recombinant Human Transmembrane protease serine 2(TMPRSS2) (R255Q),partial (Active)
Recombinant Human Transmembrane protease serine 2(TMPRSS2) (R255Q),partial (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human Transmembrane protease serine 2(TMPRSS2) (R255Q),partial (Active)
Cat：
GS13194
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Type：
Protein
Alias：
Expressed Host：
Mammalian cell
Species：
Homo sapiens (Human)
Price：
＄
Data Sheet
MSDS
  • Product details
Product Name
Recombinant Human Transmembrane protease serine 2(TMPRSS2) (R255Q),partial (Active)
Cat
GS13194
Expression Region
Uniorot ID:O15393(106-492aa (R255Q))
Alias
Expressed Host
Mammalian cell
Species
Homo sapiens (Human)
Tag
N-terminal 10xHis-tagged
Purity
Greater than 85% as determined by SDS-PAGE.
Endotoxin
Not test.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Biological activity
①Recombinant Human TMPRSS2 His tag protein (CSB-MP023924HU(M)b0) enzyme activity is measured by its ability to cleave fluorogenic peptide substrate(Boc-Gln-Ala-Arg-AMC), The Km is 19.16μM. ②Measured by Bromhexine Hydrochloride inhibit ratio on TMPRSS2 (CSB-MP023924HU(M)b0), which can cleave fluorogenic peptide substrate (Boc-Gln-Ala-Arg-AMC). The Bromhexine Hydrochloride inhibit EC50 is 81.79-154.2μM. ③Measured by Camostat Mesylate inhibit ratio on TMPRSS2 (CSB-MP023924HU(M)b0), which can cleave fluorogenic peptide substrate (Boc-Gln-Ala-Arg-AMC). The Camostat Mesylate inhibit EC50 is 0.005877- 0.01293μM.
Storage
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Type
Protein
Purposes
For research use only
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