Recombinant Human Tumor necrosis factor(TNF),partial (Active)
Recombinant Human Tumor necrosis factor(TNF),partial (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human Tumor necrosis factor(TNF),partial (Active)
Cat:
GS13052
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Type:
Protein
Alias:
Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2
Expressed Host:
E.coli
Species:
Homo sapiens (Human)
Price:
  • Product details
Product Name
Recombinant Human Tumor necrosis factor(TNF),partial (Active)
Cat
GS13052
Expression Region
Uniorot ID:P01375(77-233aa)
Alias
Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2
Expressed Host
E.coli
Species
Homo sapiens (Human)
Tag
Tag-Free
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/µg as determined by LAL method.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.0
Biological activity
The ED50 as determined in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D is less than 0.1 ng/ml.
Storage
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Type
Protein
Purposes
For research use only