Mammalian cell expression service

Currently, although it is possible to produce eukaryotic proteins in prokaryotic expression systems, the recombinant proteins generated often exhibit adverse characteristics that may impact their efficacy, such as lacking glycosylation. Mammalian cell expression systems offer unique advantages by providing a transcription and translation environment close to the wild-type, coupled with relevant chaperones, secretion, oxidative-reductive conditions and post-translational modifications that contribute to the functionality and activity of the expressed proteins.


真核哺乳细胞表达服务

（Hunter M, et al. Curr Protoc Protein Sci. 2019）

 

We have decades of experience and expertise in transient and stable expression in eukaryotic mammalian cells. We have developed a proprietary set of vectors tailored for preferred host cell types (HEK293 cells and CHO cells), along with optimized serum-free culture systems. Through improved expression and purification strategies, we aim to further enhance the yield and cost-effectiveness of recombinant proteins.

Platform features
Efficient
Efficient

The expressed recombinant protein closely mimics the structure and function of the native protein to the greatest extent possible.

Extracellular secretion expression
Extracellular secretion expression

High yield with fully folded structure and well-defined amino acid modifications

Mature system
Mature system

Clearly designed genetic background, streamlined operational procedures

Testing conditions
Testing conditions

Cultivation temperature, induction conditions; host selection, and tag design

Data
Data

SDS-PAGE, WB analysis for quality control, Nanodrop quantification

Service Content

Cycle

Survey
Survey

Preliminary research conducted

1 day
Designing
Designing

Drafting the initial expression strategy, determining tags, vectors, and expression cell lines

1 day
Expression testing
Expression testing

Gene synthesis, vector construction, testing expression and purification conditions

4-5 weeks
Scale-up production
Scale-up production

Determine the production volume based on demand

2-3 weeks
Delivery
Delivery

Experimental reports, cloning vectors, recombinant proteins

1 day
Case Show
Target protein A

The target protein A is a cell surface protein, belonging to type I single transmembrane protein.

We selected the extracellular functional domain as the target segment for recombinant expression, optimized codons, and constructed it into the expression vector. We used the hFc fusion tag and employed HEK293 cell line for transient recombinant expression through transfection.

Target protein A
Please feel free to contact us for more information or a detailed quote！