Recombinant Human Macrophage migration inhibitory factor(MIF) (Active)
Recombinant Human Macrophage migration inhibitory factor(MIF) (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human Macrophage migration inhibitory factor(MIF) (Active)
Cat:
GS13171
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Type:
Protein
Alias:
(MIF)(Glycosylation-inhibiting factor)(GIF)(L-dopachrome isomerase)(L-dopachrome tautomerase)(Phenylpyruvate tautomerase)
Expressed Host:
Mammalian cell
Species:
Homo sapiens (Human)
Price:
  • Product details
Product Name
Recombinant Human Macrophage migration inhibitory factor(MIF) (Active)
Cat
GS13171
Expression Region
Uniorot ID:P14174(2-115aa)
Alias
(MIF)(Glycosylation-inhibiting factor)(GIF)(L-dopachrome isomerase)(L-dopachrome tautomerase)(Phenylpyruvate tautomerase)
Expressed Host
Mammalian cell
Species
Homo sapiens (Human)
Tag
C-terminal hFc-tagged
Purity
Greater than 90% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Biological activity
①Measured by its binding ability in a functional ELISA. Immobilized MIF at 2 μg/ml can bind Anti- MIF Rabbit Monoclonal Antibody(CSB-RA146975A0HU), the EC50 is 49.61-69.45 ng/ml.
Storage
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Type
Protein
Purposes
For research use only