Cat：
GS20001
Type：
Antibody
Immunogen：
GFP,eGFP,eYFP,GFP,GFP tag,YFP
Immunofluorescence staining of 293F cells transfected with GFP at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).
Reactivity：
GFP Protein
Immunoprecipitating GFP in 293F whole cell lysate transfected with GFP; For western blotting, the blot was detected with GS20001 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
Applications：
ELISA, WB, IF, FC, IP; Recommended dilution: WB:1:5000, IF:1:50-1:200, FC:1:100-1:300, IP:1µl-2µl
Price：
＄
Two-color flow cytometric analysis showing 293F cells untransfected (Left) or transfected with GFP (Right) . The cells were fixed in 70% ethanol at 4℃ overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C.