Flag Monoclonal Antibody
Flag Monoclonal Antibody
Flag Monoclonal Antibody

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Flag Monoclonal Antibody
Cat：
GS20002
Type：
Antibody
Immunogen：

DDK tag,DYKDDDDK,Flag,Flag Tag

Immunofluorescence staining of 293T cells transfected with Flag tag by GS20002 at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-mouse IgG(H+L).
Reactivity：

DYKDDDDK

Applications：

ELISA, WB, IF, IP; Recommended dilution: WB:1:1000-1:10000, IF:1:100-1:300, IP:0.5µl-2µl

Price：
＄
Western Blot: Positive WB detected in: Flag Tag fusion protein 1, 2, 3, 4 at 50ng; All lanes: Flag Tag antibody at 1:1000; Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution.
Data Sheet
MSDS
  • Product details
Product Name
Flag Monoclonal Antibody
Cat
GS20002
Heavy chain

/

Light chain

/

Isotype

IgG1

Host

Mouse

Purity

>95% by SDS-PAGE

Endotoxin

/

Immunogen

DDK tag,DYKDDDDK,Flag,Flag Tag

Reactivity

DYKDDDDK

Applications

ELISA, WB, IF, IP; Recommended dilution: WB:1:1000-1:10000, IF:1:100-1:300, IP:0.5µl-2µl

Conjugated

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300；Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage

store at -20°C or -80°C. Avoid repeated freeze.

Type
Antibody
Purposes

For research use only

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