Cat：
GS20004
Type：
Antibody
Immunogen：
HA-Tag,HA,HA tag
Immunofluorescence staining of 293F transfected cells with GS20004 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Reactivity：
YPYDVPDYA
Overlay histogram showing 293F transfected cells stained with GS20004 (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C.
Applications：
ELISA, WB, IF, FC, IP; Recommended dilution: WB:1:5000, IF:1:50-1:200, IP: 1:5000-1:100000, FC：1:50-1:200
Price：
＄
Immunoprecipitating HA-Tag in 293F transfected whole cell lysate. Lane 1: Mouse control IgG (1µg) instead of GS20004 in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: GS20004 (5µg) + 293F transfected whole cell lysate (500µg); Lane 3: 293F transfected whole cell lysate (20µg)