Proximity labeling in mammalian cells with TurboID and split-TurboID
Proximity labeling in mammalian cells with TurboID and split-TurboID
2024-10-29
TurboID is an engineered biotin ligase that uses ATP to convert biotin into biotin-AMP, an active intermediate that covalently labels neighboring proteins. Optimized through direct...
Small cell lung cancer treatment target DLL3
Small cell lung cancer treatment target DLL3
2024-10-28
Delta-Like Ligand 3 (DLL3) is a member of the Delta-like ligand (DLL) family. It is an inhibitory Notch ligand that is highly expressed in small cell lung cancer (SCLC) and other n...
Interleukin-27
Interleukin-27
2024-10-27
Interleukin-27 (IL27) is a cytokine that has significantly different effects on immune responses. IL27 stimulates T cells to promote the secretion of the anti-inflammatory cytokine...
T-cells costimulate the immune checkpoint molecule 4-1BB
T-cells costimulate the immune checkpoint molecule 4-1BB
2024-10-25
4-1BB is a T cell co-stimulatory immune checkpoint molecule also known as CD137 or TNF receptor superfamily member 9 (TNFRSF9). The interaction between 4-1BB and its primary ligand...
Epidermal growth factor receptor EGFR
Epidermal growth factor receptor EGFR
2024-10-24
Epidermal growth factor receptor (EGFR), also known as HER1 and ERBB, is a receptor tyrosine kinase. EGFR is a cell surface protein that binds to epidermal growth factor, inducing ...
Treatment of obesity and metabolic diseases target GLP1R
Treatment of obesity and metabolic diseases target GLP1R
2024-10-22
The glucagon-like peptide 1 receptor (GLP1R) is an important member of the G protein-coupled receptor (GPCR) family. It specifically interacts with GLP-1, a key hormone that plays ...
How to isolate channel-forming membrane proteins using the E.coli expression system
How to isolate channel-forming membrane proteins using the E.coli expression system
2024-10-21
BackgroundRecombinant expression, isolation, and characterization of perforins are among the most common strategies used to understand their membrane permeability properties. One t...